NASC Stock Detail Page
NASC ID: N850196
Name: WiscDsLox286D01
ABRC stock number: CS850196
Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line. Associated Polymorphisms: Wiscseq_DsLox286D01.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G27720.1 (chr 2 pos 11818054 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG797278. Wiscseq_DsLox286D01.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G35880-P355 and AT5G35890.1 (at chr 5 pos 14024632 (C/14024632-14024714) on the TAIR10). The sequences' 1-84 bps mapped on the genome, 84-100 bps onto the T-DNA. Pools P 1, R 1, C 4 N 3. The insertion site is at the 3' end of the sequence mapping. Genbank ID: KO453300. Wiscseq_DsLox286D01.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G37030.1 and AT5G37040.1 (at chr 5 pos 14638495 (C/14638495-14638581) on the TAIR10). The sequences' 1-87 bps mapped on the genome, 85-99 bps onto the T-DNA. Pools P 1, R 1, C 4 N 3. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO453594. Wiscseq_DsLox286D01.3 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G56390.1 and AT5G56400.1 (chr 5 pos 22845181 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG797279.
Donation Date: 2004-12-01
| Donated by: | University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman |
Donor Number: WiscDsLox286D01
Part of : Wisconsin DsLox T-DNA lines
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available to order at NASC | £11.00 |
Germplasm Info
Mutagen: T-DNA Insertion
Background: Col-2
Transformation method: Agrobacterium
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
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